Objective To observe the effects of pretreatment with transcutaneous trigeminal nerve electrostimulation ( TNS ) on expression of glutamic acid decarboxylase( GAD )65/67 in rat hippocampus with pentetrazol-induced seizures and explone its possible anti-epileptic mechanisms. Methods Rats were divided into control group and TNS group which were given pentetrazol( PTZ ) after I ,7.14,28 d consecutive electrostimulation respectively, and subsequently the severity of seizure was quantitatively evaluated within 2 h after intraperitoneal injection of PTZ. The GAD65 and CAD67 protein was analyzed by immunohistochemistry at different time quantitatively in the regions of hippocampus. Results ① Compared with the corresponding control group, the severity of seizure in 14 d and 28 d consecutive TNS groups was significantly milder ( P ＜0. 05 ), while in I d and 7 d groups, little but no significant change was observed ( P＞0. 05 ).② Compared with the corresponding control group, the number of GAD65 posi tive cells in hippocampus was significantly increased in the 7 , 14 ,28 d TNS groups ( P 0.05),TNS组连续刺激14、28 d大鼠癫痫发作程度明显减轻(P0.05),TNS组7、14、28 d大鼠海马区GAD65表达数目明显增加(P<0.05),并且随刺激时间延长,GAD65阳性细胞增加越明显.③ 与相应时间点对照组比较,TNS各组GAD67阳性细胞光密度差异均有统计学意义(P<0.05),但表达增加与刺激时间长短无明显关系.结论 经皮TNS预处理对癫痫发作有一定影响,可能与海马区内GAD65和GAD67阳性表达增多,进而诱导脑内抑制性机制的增强有关.
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