五乙酰栀子酰苯胺对D-GalN所致小鼠急性肝损伤的保护作用

摘要

Objective To investigated the protective effects of GP-Ac-aniline on acute liver injury induced by D-galactosamine in mice. Methods By intraperitoneal injection of D-galactosamine (D-GalN ) model of acute liver injury in mice was established. Serum alanine aminotransferase ( ALT ), aspartate aminotransferase ( AST ) activity and liver homogenate superoxide dismutase ( SOD ), malondialdehyde ( MDA ), glutathione peroxidase ( GSH-Px ), protein content, and liver index were measured, and HE staining was used to observe the morphological changes of liver pathology. Results The GS-Ac-aniline dose groups could decrease serum ALT and AST activity, improve liver homogenate SOD, GSH-Px activity, reduce the liver homogenate MDA and protein content, and reduce the liver index as well. The reduced serum ALT and AST activity, liver homogenate MDA content and decreased liver index were statistically different ( P <0. 05 or P <0. 01 ). Although the improvement of liver homogenate SOD, GSH-Px and protein content level were not statistically significant, there were different degrees of improvement ( When GP-Ac-aniline at the dose of 150 mg/kg, liver homogenate GSH-Px and protein content level had significant difference ). The medium, high-dose groups of GS-Ac-aniline ( 100,150 mg/kg ) could significantly improve the degree of liver injury. The GS-Ac-aniline dose groups could reduce liver pathological damage observed under light microscopy. Conclusion GP-Ac-aniline can improve the acute liver injury induced by D-galactosamine, and the mechanism might be related to the antioxidant process.%目的 探讨五乙酰栀子酰苯胺对D-氨基半乳糖(D-GalN)所致小鼠急性肝损伤的保护作用.方法 采用D-GalN腹腔注射法建立小鼠急性肝损伤模型,生化法检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性和肝匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽-过氧化物酶(GSH-Px)、蛋白含量以及肝脏系数,HE染色观察肝脏病理形态学改变.结果 五乙酰栀子酰苯胺剂量 (50、100、150 mg/kg) 组能降低血清ALT、AST活性,提高肝匀浆SOD、GSH-Px活性,降低肝匀浆MDA及蛋白含量,亦能降低肝脏系数.其中降低血清ALT、AST活性、降低肝匀浆MDA含量及降低肝脏系数具有统计学意义(P＜0.05或P＜0.01),虽然对肝匀浆SOD、GSH-Px及蛋白含量水平的作用差异无统计学意义,但是也有不同程度的改善(其中五乙酰栀子酰苯胺剂量为150 mg/kg时对肝匀浆GSH-Px及蛋白含量水平的作用差异有统计学意义).五乙酰栀子酰苯胺中、高剂量 (100、150 mg/kg) 组能明显改善肝组织损伤的程度,光镜下显示五乙酰栀子酰苯胺剂量组肝脏病理损伤有所减轻.结论 五乙酰栀子酰苯胺对D-GalN所致小鼠急性肝损伤具有改善作用,其作用机制可能与抗氧化有关.