Objective To investigate the anti-cancer effect of CT-1042 on human gastric cancer cells SGC-7901 in vitro. Methods The inhibitory effect of CT-1042 on proliferation of SGC-7901 cells was evaluated by MTT method, Hoechst 33342 staining was performed to observe the influence of CT-1042 on cell apoptosis morphology. The cell apoptosis rate was detected by Annexin V and PI double dye. JC-1 probe was conducted to detect the CT-1042 effects on mitochondrial membrane potential. Flow cytometry was used to determine the cell cycle distribution. Results CT-1042 could significantly inhibit the proliferation of the subject cells, and induce the nuclear fragmentation, chromatin shrinkage. CT-1042 could induce subject cells mitochondrial membrane potential decreased, lead to cell apoptosis. CT-1042 could arrest the cycle progression of the subject cells in the G2/M phase. Conclusion CT-1042 has a significant inhibitory effect on human gastric cancer cell SGC-7901 in vitro, and the mechanism underlying may contribute to induce cell apoptosis and cycle arrest.%目的 探索CT-1042体外对人胃癌细胞SGC-7901的抗癌效应.方法 采用MTT法测定CT-1042对SGC-7901细胞的增殖抑制效应;Hoechst 33342染色观察CT-1042对细胞凋亡形态的影响;Annexin V和PI双染检测CT-1042对细胞凋亡率的影响;JC-1探针检测CT-1042对细胞线粒体膜电位的影响;流式检测CT-1042对细胞周期分布的影响.结果 CT-1042对受试细胞增殖具有良好的抑制作用,并能引起细胞核碎裂、浓缩;CT-1042能够诱导受试细胞线粒体膜电位下降,导致细胞凋亡;CT-1042能够抑制受试细胞的周期进展,使之停滞于G2/M期.结论 CT-1042体外对人胃癌细胞SGC-7901具有很好的抑制作用,并能诱导其发生细胞凋亡和细胞周期阻滞发挥抗肿瘤作用.
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