Method of in vitro immunoassay for detection of the presence of liver cancer cells in an individual method for classification of liver cancer cells present in an individual.In vitro method for determining to administer or not an anticancer agent containing an antibody anti glipicano 3 to an individual.And in vitro method for determination of a dose of an anticancer agent containing an antibody anti glipicano 3 in the treatment of liver cancer in an individual

摘要

The present invention relates to an in-vitro immunoassay method for detecting the presence of liver cancer cells in a subject. In the method of the present invention, antigen retrieval treatment based on heat-induced epitope retrieval method and antigen retrieval treatment based on protease-induced epitope retrieval method can be combined in the detection of glypican 3 antigen expression in liver cancer tissues to thereby detect the difference in the expression level or expression pattern of the glypican 3 antigen by immunohistochemical staining method. This enables samples, which has been determined by the conventional HIER method as highly expressing glypican 3, to be graded according to the expression level of glypican 3.