低氧对高原鼠兔和大鼠血液NO与ET-1的影响

摘要

The aim of the present study was to compare the effects of hypoxia on NO,ET-1 and NO/ET-1levels in Wistar rats and pikas. We determined NO and ET-1levels in the serum of 30 rats (three groups of 10 rats, each taken to 3 780 m and divided into 24 h, 2 wk and 3 wk durations) and 10 pikas at 3 780 m by ELISA and Nitrate Reductase Method. We compared the values of NO, ET-1 and NO/ET-1 in rats of 24 h duration with those in rats of 2 wk and 3 wk durations.We then compared NO, ET-1 and NO/ET-1 values in the 24 h, 2 wk, 3 wk groups with those in pikas. In the beginning of entry to high altitude, the NO and ET-1levels in rats were markedly increased compared to those in pikas (respectively, P＜0.01), whereas the values of NO/ET-1in rats and pikas were very close (P＞0.05). There was a decreasing trend in NO levels and an increasing trend in ET-1levels (respectively, P＜0.01) with passage of time at high altitude in rats, and NO was well correlated with ET-1(r2=0.2416, P＜0.01). The NO/ET-1levels in pikas was approximately 2 fold greater than in the 2 wk and 3 wk groups (P＜0.01). In conclusion, NO,ET-1and NO/ET-1levles in Wistar rats and pikas at 3 780 m are very different, may indicate adaption or acclimitization to high altitude hypoxia.%将Wistar大鼠暴露于3 780 m低氧环境,分别于24 h、2 wk及3 wk后采用酶联免疫法和硝酸还原酶法测定血液中的ET-1和NO的含量,计算NO/ET-1值,并与高原鼠兔比较,探讨低氧条件下大鼠与高原鼠兔血液中NO与ET-1含量的变化趋势.结果表明,低氧24 h后,大鼠血液中NO和ET-1的含量显著高于同海拔的高原鼠兔(P＜0.01),而NO/ET-1值无显著差异(P＞0.05).随着大鼠在高海拔停留时间的延长,血液中NO含量呈减少趋势,而ET-1则有上升趋势,二者呈显著的负相关(r2=0.2416, P＜0.01).高原鼠兔NO/ET-1值约为大鼠低氧2 wk和3 wk的2倍(P＜0.01).说明不同低氧暴露时间,高原鼠兔和大鼠的NO、ET-1及NO/ET-1值有显著差异,提示NO/ET-1值可以作为有机体是否适应高原低氧环境的一个指标.