葡萄叶片中营养物质和叶绿素含量与其对绿盲蝽抗性的关系

摘要

[目的]研究葡萄品种的抗性对于绿盲蝽Apolygus lucorum综合防控策略的制定具有重要意义.本研究检测了不同葡萄品种对绿盲蝽的抗性水平,分析了叶片中可溶性蛋白、可溶性糖和叶绿素含量与其对绿盲蝽抗性的关系,以明确不同葡萄品种对绿盲蝽的抗性机理.[方法]以9个葡萄品种(巨峰、玫瑰香、红地球、红宝石、藤稔、赤霞珠、蛇龙珠、霞多丽和品丽珠)为材料,通过连续3年的田间抗性鉴定确定这些葡萄品种对绿盲蝽的抗性水平;分别采用考马斯亮蓝法、蒽酮比色法和乙醇提取法测定不同葡萄品种叶片中可溶性蛋白、可溶性糖和叶绿素的含量,并通过线性回归法分析这些物质含量与葡萄对绿盲蝽抗性的关系.[结果]不同葡萄品种对绿盲蝽的抗性水平不同,按抗性指数从小到大依次为红地球、玫瑰香、巨峰、赤霞珠、品丽珠、红宝石、蛇龙珠、藤稔和霞多丽;不同葡萄品种叶片中可溶性蛋白、可溶性糖和叶绿素含量存在差异;可溶性蛋白和叶绿素含量与抗性指数之间没有显著相关性,而可溶性糖含量与抗性指数之间存在显著负相关(P＜0.05).[结论]不同葡萄品种对绿盲蝽的抗性存在显著差异,藤稔和霞多丽对绿盲蝽具有较高抗性,而红地球、玫瑰香和巨峰则较为敏感,该差异可能与葡萄叶片中可溶性糖的含量有关.%[Aim] Studying the resistance of grape varieties is very important for developing the integrated pest management of the green plant bug,Apolygus lucorum.In this study,the resistance levels of different grape varieties were identified,and the relationship between the contents of soluble proteins,soluble sugars and chlorophylls in grape leaves and their resistance to A.lucorum was analyzed,so as to explore the resistance mechanism of different grape varieties to A.lucorum.[Methods] The resistance levels of nine grape varieties (Kyoho,Muscat Hamburg,Red Globe,Ruby,Fujiminori,Cabernet Sauvignon,Cabernet Gernischt,Chardonnay and Cabernet Franc) were tested in the field for three consecutive years,the contents of soluble proteins,soluble sugars and chlorophylls in grape leaves were determined with the coomassie brilliant blue method,anthrone colorimetry method and ethanol extraction method,respectively,and the relationships between the contents of these substances and their resistance to A.lucorum were analyzed with linear regression method.[Results] The resistance levels of different grape varieties to A.lucorum were obviously different.The resistance indices of the varieties Red Globe,Muscat Hamburg,Kyoho,Cabernet Sauvignon,Cabernet Franc,Ruby,Cabernet Gernischt,Fujiminori and Chardonnay increased sequentially.The contents of soluble proteins,soluble sugars and chlorophylls in different grape varieties were different.Correlation analysis showed that the contents of soluble proteins and chlorophylls in grape leaves had no correlation with the resistance index,while the content of soluble sugars had a significantly negative linear correlation with the resistance index (P ＜ 0.05).[Conclusion]Different grape varieties show different resistance levels to A.lucorum.Fujiminori and Chardonnay have high resistance to A.lucorum,while Red Globe,Muscat Hamburg and Kyoho have low resistance to A.lucorum.This difference of resistance to A.lucorum may be related to the content of soluble sugars in grape leaves.