棘托竹荪液态深层发酵水提物和不同浓度乙醇提取物的抗氧化活性及化学成分分析

摘要

测定棘托竹荪(Dictyophora echinovolvata)液体发酵菌丝体和发酵液水提物及不同浓度乙醇提取物DPPH自由基清除能力以评价其抗氧化活性。结果表明：棘托竹荪菌丝体的20%、60%乙醇提取物，发酵液的20%、60%和95%乙醇提取物均具有较好的抗氧化能力，其清除DPPH自由基的能力强于对照品芦丁，其中棘托竹荪发酵菌丝体的20%醇提物抗氧化活性最强，其清除 DPPH 自由基的 IC50值为0.012 mg/mL。利用中药材系统预实验方法鉴定了棘托竹荪发酵菌丝体和发酵液水提物及不同浓度乙醇提取物所含化学成分类型。结果表明：棘托竹荪发酵菌丝体和发酵液的水提物及不同浓度乙醇提取物中均可能含有糖类、氨基酸和蛋白、有机酸等物质，而黄酮体、生物碱、蒽醌和甾体等类物质在不同浓度乙醇提取物中的含量有所差异。%Antioxidant activity of aqueous and ethanolic (20%,60% and 95%)extracts of Dictyophora echinovolvata mycelium and spent culture broth was determined using the DPPH free radical-scavenging assay.Mycelial extracts prepared with 20% and 60% EtOH,and material extracted from spent culture broth using all three EtOH concentrations,exhibited higher antioxidant activity compared with rutin controls. Highest overall antioxidant activity (IC50 value = 0.012 mg/mL)was recorded with material extracted from D.echinovolvata mycelium with 20%EtOH.Qualitative determination of the major chemical components of each extract sample was carried out using Traditional Chinese Medicine experimental methods.All the extracts contained sugars,organic acids,amino acids and protein,but there was considerable variation in the flavonoid,alkaloid,anthraquinone and steroid distribution patterns.