微波辅助乙醇/硫酸铵双水相提取分离蛹虫草发酵产物中虫草素和虫草酸的研究

摘要

Microwave-assisted aqueous two-phase extraction technology was applied to extract and separate cordycepin and cordycepic acid from Cordyceps militaris mycelium grown in submerged culture.The optimal phase composition was ethanol and (NH4)2 SO4 with a mass fraction of 25％ and 22％,respectively.Orthogonal testing determined the optimal microwave assisted extraction conditions to be:microwave power 540 W,microwave exposure time 120 s,solid to liquid ratio 1 ∶ 100 [mycelium dry weight (g):weight of aqueous two-phase extraction system (g)].Under these conditions,cordycepin and cordycepic acid extraction yields were (99.35 ± 0.12)％ and (99.02 ± 0.16)％,respectively,and the percentage distribution of cordycepin in the upper phase and cordycepic acid in the lower phase were (95.24 ± 0.25) ％ and (91.83 ± 0.19)％,respectively.The high extraction yields and good separation of cordycepin and cordycepic acid achieved should facilitate subsequent purification operations.%采用微波辅助乙醇/硫酸铵双水相提取分离蛹虫草(Cordyceps militaris)发酵产物中的虫草素和虫草酸.确定了双水相系统中乙醇质量分数为25％、硫酸铵质量分数为22％.正交试验优化的微波提取条件为微波功率540 W,提取时间120 s,固液比1∶100[菌丝体干重(g):双水相体系的质量(g)].在此条件下,虫草素和虫草酸的提取率分别为(99.35±0.12)％和(99.02±0.16)％,虫草素在上相中的分配率为(95.24±0.25)％,虫草酸在下相中的分配率为(91.83±0.19)％.该方法提取率高,虫草素和虫草酸分离效果好,有利于产品的后续纯化.