藏药蓝侧金盏花的挥发油成分分析及其抑菌活性研究

摘要

The volatile oil of Adonis coerulea Maxim was extracted by steam distillation and analyzed by GC-MS.The antibacterial activity of the volatile oil was studied by filter paper diffusion method.The results showed that there were 34 chemical constituents with over 85％ matching degree of Adonis coerulea Maxim by GC-MS,which accounted for 64.17％ of relative content of total ion peak,and the main compositions included carene (9.70％),alpha-pinene (5.70％) and alpha-bisabolol (4.82％).The volatile oil of Adonis coerulea Maxim had good inhibitory activity on 7 kinds of gram-negative bacteria and 1 gram-positive bacteria,and had no inhibitory effect on the 3 tested fungi.%采用水蒸气蒸馏法获得藏药蓝侧金盏花的挥发油,对其进行GC-MS分析,并运用滤纸片扩散法初步研究了该挥发油的抑菌活性.结果表明:对蓝侧金盏花挥发油GC-MS分析得到34种匹配度均在85％以上的化学成分,占总离子峰相对含量的64.17％,其主要成分包括蒈烯(9.70％)、α蒎烯(5.70％)和红没药醇(4.82％).纸片扩散法测试藏药蓝侧金盏花挥发油对7种革兰氏阴性菌和1种革兰氏阳性菌有较好的抑制活性,对3种供试真菌无抑制作用.