Human keratinK14 andK5 are produced in stratified epithelia. This tissue-specific expression is directed by the two promoters which have been used extensively to drive the expression of transgenes to the skin and hair follicle. In this paper, the pGL3-K14 and pGL3-K5 promoter vectors were constructed by insert the K14 and K5 promoter into the MCS of pGL3-Basic, respectively. The promoters activity was analyzed by two luciferase reporter assay and GFP expression test. These results showed that two promoters were both expressed in all cell lines. The activity of K14 promoter was higher in skin cell line than in others cell lines.%角蛋白14(K14)和角蛋白5(K5)主要在毛囊基质和复层鳞状上皮基底层的角质细胞里表达.这两个启动子已被广泛应用于毛囊特异表达外源基因的转基因小鼠模型的研究中,为了能将其应用于转基因绵羊生产,探讨基因对绵羊毛囊发育的影响,从人基因组中克隆得到K14和K5启动子,利用不同的报告基因在细胞水平分析各启动子的表达活性.结果表明,这两个启动子在不同细胞系中都能驱动外源基因的表达,而且K14启动子在小鼠皮肤细胞系JB6-C41中的表达活性高于其他细胞.
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