Construction of the PD-L1 Promoter-Luciferase Reporter Expressing Vector for Small Molecule Inhibitors Screening

摘要

T cell mediated immune response plays an important role in modulating the growth, proliferation and recurrence of tumor cells. Activation of T cell requires binding of the T cell receptor (TCR) with a cognate peptide presented on the MHC of an APC and a co-stimulatory signal, mainly generated between members of the B7 ligand family on the APC and the CD28 receptor family on the T cell. On the contrast, the inhibitory signaling between these two families acts to down-grade T cell activation, resulting in T cell exhaustion, deletion or tolerance [1]. Antigen-specific T cell responses are regulated by the balance between co-stimulatory and co-inhibitory signals [2, 3], and modulation of such co-signal pathways is beneficial when immune intervention is necessary [4].