原花青素对体外阿尔茨海默病的预防作用

摘要

Objective:To investigate the preventive effects of proanthocyanidins( PC) on Alzheimer′s disease in vitro.Methods:Alzheimer′s disease was mimicked in vitro by PC12 cell line injury induced with H 2O 2(terminal concentration,100 μmol/L)after incubation for 18 h.The cell lines were pre-trea-ted with PC in dose of 80 mg/L, 40 mg/L and 20 mg/L as well as Tempol (800 μmol/L,positive control) 30 min before modeling.ELISA was performed to detect the content of Trx in the cellular supernatant.Xanthine oxidase method was used to determine the T-SOD activity of cells,and thiobarbituric acid was used for measuring the content of MDA.Results:After induction of the PC12 cell injury with H2O2 in dose of 100μmol/L,the cellular Trx content and T-SOD activity were markedly decreased,whereas the content of MDA was increased significantly(P<00.1).However,treatment with PC in different dose had increased the content of Trx and boosted T -SOD activity,yet reduced the content of MDA in PC12 cell lines(P<0.01,P<0.05).Conclusion:Proan-thocyanidins may alleviate the oxidative damage in PC12 cells induced with H2 O2 .%目的：研究原花青素（ PC）对体外阿尔茨海默病模型（ AD）的预防作用。方法：H2 O2100μmol/L诱导PC12细胞损伤18 h复制体外阿尔茨海默病模型，原花青素80 mg/L、40 mg/L、20 mg/L、Tempol 800μmol/L于造模前30 min预处理后，双抗体一步夹心法酶联吸附实验（ ELISA）检测细胞上清液中硫氧还蛋白（ Trx）含量；黄嘌呤氧化酶法检测细胞中的总超氧化物歧化酶（ T-SOD）活力；硫代巴比妥酸盐法测定细胞上清液的丙二醛（ MDA）。结果：H2 O2100μmol/L诱导PC12细胞损伤后，细胞内Trx浓度和T-SOD活力显著降低，MDA含量显著升高（P＜0．01）；原花青素各剂量组可增高Trx浓度和T-SOD活力，降低MDA含量（P＜0．01，P＜0．05）。结论：原花青素能减轻H2O2引起PC12细胞的氧化应激损伤。