Heterologous expression of bovine lactoferricin in Escherichia coli

摘要

According to the bias of codon utilization of Escherichia coli, a fragment encoding bovine lactoferricin(LfcinB) has been cloned and expressed in the Escherichia coli under the control of the T7 promoter. The target peptide was expressed by IPTG induction, purified by affinity chromatography with 6XHis tagged resin. The recombinant bovine lactoferricin appears to be successfully expressed, as it displays antibacterial activity (antibacterial assay). Moreover, the identity of the recombinant product was estimated by Tricine-SDS-PAGE.