Detection of Single Nucleotide Polymorphisms Associated with Venous Thrombosis Using Multiplex Miniseqencing Reaction

摘要

Venous thrombosis (VT) is a multifactural disease with an interplay of acquired and genetic thrombotic risk factors, In this paper we describe technique referred to as a multiplex minisequencing reaction, which consist of analyses of four single nucleotide polymorphism (SNP) that predispose to thrombosis. Hereditary risk factors detected by the multiplex minisequencing reaction include G1691A mutation in factor V gene cold Factor V Leiden (FVL), prothrombin gene G20210A mutation and MTHFR (methylentetrahydrofolate reductase) gene C677T and A1298C mutation.