ABERRANT MIRNA EXPRESSION RESPONSE TO UV IRRADIATION IN HEPG2 CELLS

摘要

MicroRNAs (miRNAs) are small, highly conserved, noncoding RNA that regulate gene expression at the posttranscriptional leveL Expression of these small RNA genes is fine regulated during development, differentiation and apoptosis of normal cells, but they are often deregulated in various cancers. miRNA expressions are also affected by cellular stress, such as radiation, chemotherapy and so on. In this research, we monitored the expression levels of several miRNAs by employing the stem-loop real-time polymerase chain reaction (PCR) in HepG2 cells after UV-radiatioa Our data showed that ultraviolet irradiation can induce the change of miRNA expression levels in HepG2 cells. Among them miR-26a, miR-34a miR-146a were up-regulated significantly, while the expression of the miR-21 was down-regulated significantly. Bioinformatics analysis of these significantly regulated miRNAs was discussed. The results also indicate that miRNAs might be part of the innate response mechanism of the cells to radiation injury.