Rapid and selective screening method on peptaibiotics/peptaibols using LC-ESI-MS

摘要

Peptaibiotics are defined as a family of fungal peptides containing a high proportion of the nonproteinogenic amino acid Aib(alpha-aminoisobutyric acid)and exerting biological activities.N-acetylated members of this group containing a C-terminal 1,2-amino alcohol are defined as peptaibols.Lipopeptaibols are acylated with a fatty acid at the N-terminus,and aminolipopeptides contain unusual heterocyclic residues at N-and/or C-termini.As nowadays more than 300 peptaibiotics are known reliable methods are needed that enable the rapid differentiation among new and already known peptides.Here we present a screening method,applicable directly on filamentous fungi grown on agar plates,combining solid-phase extraction with HPLC-ES1-MS.By comparing partial sequences deduced from analytical data with those compiled e.g.in the "Peptaibol-Database",the judgement is possible whether or not structures are novel,identical or related to structures already known.