Interaction of cadmium-cysteine binding and oxidation of protein causes of blood thrombosis

摘要

Thrombosis occurs if the balance between thrombogenic factors and protective mechanisms is disrupted. These disorders can be caused by endothelial disorders due to the presence of heavy metals such as cadmium (Cd). These amino acid residues can bind Cd metal covalently with the -S-S-, -C-S-, -SH, and phenyl groups, which are present in the amino acid residues. This situation causes changes in the structure of the protein, so that it becomes modified and forms a blood clot (thrombosis). However, the spectroscopic interaction of cadmiumcysteine as causes of thrombosis has not been widely studied. Therefore, this research needs to be done. The research conducted was an experimental study with posttest control with group design. This study used healthy blood obtained from PMI Banjar regency. This study used one control group and three treatment groups, namely P0 = control group, blood without Cd; P1 = blood group with Cd 0.003 mg/l; P2 = blood group with Cd 0.03 mg/l; P3 = blood group with Cd 0.3 mg/l; P4 = blood group with Cd 3 mg/l. Furthermore, each group was incubated for 45 minutes at 37°C. Then cloth weight, AOPP levels, and absorbance were measured at wavelengths 253 nm. The results showed that an increase in cadmium levels caused an increase in the rate of thrombosis. This increase is associated with an increase in AOPP levels. Cadmium can bind covalently with cysteine and other amino acids which results in the breakdown of peptide bonds. The study concluded that cd accelerated the rate of thrombosis due to increased protein oxidation (aopp). This oxidation is caused by binding cd to cysteine with kbind = 0.355.