A Preliminary Study on the Antioxidant Activity of Selenoprotein in Cordyceps Militaris Rich in Selenium

摘要

Utilize different solvents to extract and salt out soluble protein from the Cordyceps rich in selenium which is artificially cultivated, thus obtaining different types of selenoproteins contained in it, and then pyrogallol autoxidation method is applied to determine their antioxidant activity, meanwhile, double-channel atomic fluorescence is used to detect the materials and their selenium content. The results show that: various proteins of Cordyceps rich in selenium all contain selenium, followed by alkali-soluble selenoprotein > alcohol-soluble selenoprotein > salt-soluble selenoprotein > water-soluble selenoprotein; their ability to remove superoxide anion is followed by water-soluble protein> alkali-soluble protein > salt-soluble protein> alcohol-soluble protein, the clearance rate of selenoprotein with different salting points on superoxide anion is shown as follows: salting selenoprotein with the rate of 70% > salting selenoprotein with the rate of 100% > salting selenoprotein with the rate of 90% > salting selenoprotein with the rate of 80%> salting selenoprotein with the rate of 50%> salting selenoprotein with the rate of 60%; therefore, selenoprotein of Cordyceps rich in selenium is a kind of natural resource with good antioxidant activity as well as broad application prospects.