Highly Efficient, Automated miRNA Isolation Method Using Solid Phase Reverse Immobilization (SPRI) Technology and Biomek - (PPT)

摘要

Changing the binding, wash and rebinding conditions allow to capture small nucleic acid at 22 nucleotides. Both manual and Biomek Liquid Handler methods showed similar yield for the 22 nt oligonucleotides. Expression of the let-7c miRNA in the TaqMan assay demonstrated that we have successfully isolated the miRNA from HeLa cells using the Agencourt RNAdvance Cell v2 kit and miRNA from FFPE samples using the Agencourt FormaPure kit. Biomek NX-MC method (PN# A47946) is available for cell miRNA isolation. Biomek Span8 method (PN# A35556) is available for FFPE miRNA isolation.