Automated miRNA Isolation Using SPRI: Changing Binding Conditions Allows for Recovery from Cell Culture, FFPE, andTissue Samples

摘要

There is great interest in understanding how miRNA can be used as a biomarker for cancer development and cancer prognosis, and discovering how it might be used as a therapeutic target for cancer treatment. With high demand for, and the fast growth of,miRNA research, an efficient and robust miRNA purification method was needed to enhance downstream assays. Most nucleic acid extraction procedures do not favor miRNA recovery, as smaller nucleic acids such as miRNA are removed during purification processes. In this article, two methodologies for recovering miRNA using the Agencourt SPRI chemistry (Beckman Coulter Life Sciences; www.beckman.com) are described.