Preparation of Eucommia ulmoides Leaves Antioxidant and Its Antioxidation Activity in Vitro

摘要

The adsorption and desorption efficiencies on flavonoids and polyphenols from Eucommia ulmoides leaves by different resins were investigated, and the most suitable resin for preparation of Eucommia ulmoides leaves antioxidant was chosen. Then the crude extract of Eucommia ulmoides leaves was adsorbed by resin, and the resin was eluted by 20%, 40%, 60% and 80% (in volume) ethanol elution solution, and then the antioxidants were gotten. The flavonoids and polyphenols in the antioxidants were tested for content. The antioxidation activity in vitro was studied by testing the scavenging capacities on HO, (O_2)~- and DPPH. The result showed: the 20%, 40% and 60% ethanol elution solution had good scavenging capacities on HO, (O_2)~- and DPPH, and there was a good correlation between the contents of flavonoids and polyphenols and scavenging efficiency on each free radical; The antioxidation activities in vitro of the antioxidants was related to flavonoids and polyphenols, and the polyphenols was the leading working material; there was more polyphenols in 20% ethanol elution solution, and more flavonoids in 60% ethanol elution solution, so different antioxidant on different component content could be gotten by collecting different elution solution.