Hypoxia mediated up-regulation of DLL4 in A549 cells and its influence on migration of endothelial cells

摘要

More people died from lung cancer than any other type of cancer in China. Recently, studies have shown that antiangiogenesis is a promising therapy strategy in lung cancer treatment. Delta-like ligand 4 (DLL4), is one of the highly conserved notch ligand which participates in the process of vascular formation. Therefore, more and more attention had paid to this ligand as a cancer therapeutic target. However, the underlying evidence is still largely absent. In the present study, we intend to elucidate the effect of tumor-derived DLL4 on the migration of endothelial cells. Using cobalt chloride CoCl_2 to mimic the hypoxia condition in lung cancer cell line A549 in vitro and explore the expression of DLL4 in A549 cells. We also observe the migration of endothelial cells in the condition of CoCl_2 treated cancer cell culture supernatant.