Identification of multi-copper oxidase enzymes from LAB able to degrade biogenic amines

摘要

The main objectives of this study were the search for enzymatic activities responsible for biogenic amines (BAs) degradation in lactic acid bacteria (LAB) strains isolated from wine, their identification, and the evaluation of their applicability for reducing BAs in wine. From seventy-six LAB cell extracts, 53% showed activity against a mixture of histamine, tyramine and putrescine when were analyzed in-gel. The quantification of the degrading ability for each individual amine was tested in a synthetic medium and wine. Most of the bacteria analyzed were able to degrade the three amines in both conditions. The highest percentages of degradation in wine were those of putrescine: up to 41% diminution in-one week. Enzymes responsible for amine degradation were isolated and purified from Lactobacillus plantarum J16 and Pediococcus acidilactici CECT 5930 strains, and were identified as multicopper oxidases (MCOs). This is the first report of an efficient BA reduction in wine by LAB. Furthermore, the identity of the enzymes involved has been revealed.