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Characterization of Intercultivar Variation on the Linear Viscoelastic Network Properties of Wheat Gluten II: Effects of Temperature and L-Cysteine

机译:小麦面筋三粘弹性网络性能的跨学型变异的特征:温度和L-半胱氨酸的影响

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Both non-covalent and disulfide bonds are known to be important contributors to the unusual viscoelastic properties of wheat gluten, but it has been difficult to decouple their effects on such. In this work, the effects of added L-cysteine and an increase in temperature on the stress relaxation behavior of three glutens with different high molecular weight subunit composition were compared. An increase in temperature up to 40°C decreased the apparent network strength for all of the glutens, but the decrease in viscoelasticity varied between the glutens w ith d ifferent s ubunit c omposition. 11 w as found that addition of 250 ppm L-cysteine at 25°C to these glutens eliminated most evidence of a network structure in their stress relaxation patterns, but the resulting extended power law stress relaxation curves did not superpose themselves at 25 °C. These results suggest that use of the linear viscoelastic network strength of gluten shows promise as a new tool for assessing the intercultivar variations in gluten network structures.
机译:已知非共价和二硫键均为小麦麸质的不寻常粘弹性的重要贡献者,但难以将它们的作用脱钩。在这项工作中,比较了添加L-半胱氨酸的效果和温度升高对具有不同高分子量亚基组合物的三种子蛋白的应力松弛行为。温度升高至40°C会降低所有面子的表观网络强度,但粘弹性的降低变化在紫外线之间的ubUnit C Otposition之间变化。如图11W所示,发现在25℃下添加250ppm L-半胱氨酸,这些蛋白酶在其应力松弛模式下消除了网络结构的最多证据,但由此产生的扩展功率法应力松弛曲线在25°C下没有叠加。这些结果表明,使用麸质的线性粘弹性网络强度显示了承诺作为评估麸质网络结构的跨学生变化的新工具。

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