Production of Human Monoclonal Antibodies Against FC Epsilon Receptor I Alpha by Combining In Vitro Immunization with Phage Display

摘要

Monoclonal antibodies (mAbs) have a great potential and hope for the treatment of various human diseases. The advent of mAbs was done using hybridoma technology, which was introduced by Kohler and Milstein. However, owing to their murine origins, thefirst generation of mAbs evaluated in the clinic was limited by their immunogenicity. Thus, increasing effort has been applied to engineer antibodies without a non-human structure to minimize such responses. To overcome this problem, we developed an in vitro immunization (IVI) protocol, which elicits antigen-specific immune responses in peripheral blood mononuclear cells (PBMC). In antibody engineering, cell surface antigens are often targeted as they play an increasingly important role. However the generation of antibodies against cell surface antigens is hard owing to the difficulty in obtaining sufficient amounts of antigen with high purity and in the native conformation for immunization.