The use of Ion-Mobility Mass Spectrometry for Separation and Structural Elucidation of lipids in Human Plasma. Application in Lipidomics studies

摘要

A very powerful chromatographic method for separation of lipids has been developed. With our previous chromatographic method using a HSS T3 column we were capable of analyzing approximately 185 lipids in positive ion mode and in negative ion mode approximately 65. With the presented chromatographic method using a CSH C_(18) column we could increase already these numbers to approximately 200 and 175 lipids for positive and negative ion, respectively. With HDMS~E acquisition, using drift time information from the ion mobility cell, acyl chains of lipids can be elucidated in a fast and efficient manner with the help of non-commercial software (lipidmaps.org) or commercial software (SimLipid, TransOmics). Without the mobility function this is not possible. We are currently building a database with accurate masses, retention times and acyl chain information. When this database is finished and the different lipid isomers are elucidated the amount of lipids that can be analyzed in a single run will be increased to approximately 345 and 250 for the positive and negative ion analysis, respectively. For additional elucidation (double bond localization) TAP fragmentation works in some cases, however other possibilities are being explored.