Comprehensive lipidomic analysis of human plasma using multidimensional liquid- and gas-phase separations: Two-dimensional liquid chromatography–mass spectrometry vs. liquid chromatography–trapped-ion-mobility–mass spectrometry

摘要

Highlights?RPLC×HILIC-MS method developed for untargeted lipidomic analysis of human plasma.?Method provided separation of the major lipid classes and a peak capacity of approximately 100,000.?LC-TIMS–MS method developed for lipidomic profiling of human plasma.?Two multidimensional separation platforms (LC×LC–MSvs.LC-TIMS–MS) were compared.?Main advantages and drawbacks of both approaches are discussed.AbstractRecent advancements in separation science have resulted in the commercialization of multidimensional separation systems that provide higher peak capacities and, hence, enable a more-detailed characterization of complex mixtures. In particular, two powerful analytical tools are increasingly used by analytical scientists, namely online comprehensive two-dimensiona