Comprehensive Genome-Wide Proteomic Analysis of Human Placental Tissue for the Chromosome-Centric Human Proteome Project

摘要

We believe that high-quality, extensive proteome maps are achievable within a planned 10-year period. With the well-established analytical methods, we have accomplished genome-wide proteomic analysis of placental tissue, resulting in 4,239 identified proteins that include 219 N-linked glycopeptides and 592 phosphopeptides with high confidence (FDR < 1%). Moreover, 1331 unique proteins were quantified with high confidence (FDR < 1%). In the next step, glycoproteins, phosphoproteins, and preeclampsia-specific proteins that have been identified from the initial profiling will be subjected to validation in the context of missing proteins that would fill in the predicted 20,300 gene products. With our well-established methods, protein evidence for 13 placenta-specific genes were verified. Further studies should focus on the molecular mechanism of those identified placenta-specific proteins involved in various biological systems. The analytical methods applied to this initial profiling would also contribute to the development of a standardized platform of the C-HPP. All raw data produced in the present study will be deposited in the public database and shared with all C-HPP teams.