Biodiversity of highly-glycosylated plant phosphosphingolipids: structure determination by tandem mass spectrometry

摘要

Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, but the function of this complex family of compounds remains largely unknown. Therefore, the structural characterization of GIPC represents a critical step toward the understanding of their physiological functions and their role in membrane organization. In this work, GIPCs have been purified from a wide variety of plants tissues or algae (23 species from various phylogenetic groups, i.e. 3 species coming from algae, 2 from moss, 1 from fern, 3 from gymnosperm, 4 from monocot and 10 from dicot), yielding multiple molecular species that have been analyzed by tandem mass spectrometry, using a combination of MALDI- and ESI-MS/MS.