Identification of Phospholipids in MTBE Extract of Mouse Brain by Microfluidic Chip-based Mass Spectrometry

摘要

The mass spectra of lipid extract acidified by a acetic acid indicate very accurate without salt-adducted peaks, representing exclusively neutral polar lipids such as PC and SM. The shotgun lipidomics, two-dimensional mass spectrometric analysis which was composed of full MS and PIS (and/or NLS) was developed for identification of individual lipid molecular species. Lipid searching program with which database include more than 30,000 lipid species was also developed. 99 molecular species of lipids were identified by searching the high resolution mass spectral profiling with 'iLipid' lipid searching engine. Among them, 82 molecular species of lipid, including 23 species of PC and its subclasses, 23 of PE and its subclasses, 8 of PA, 8 of PG, 9 of PS, 4 of PI and 7 of ST were confirmed by using a intrasource separation method on triple quadrupole and Q-TOF mass spectrometers interfaced with nanomate device.