Method for inactivating and extracting mycobacteria from liquid media for characterization and / or identification using mass spectrometry

摘要

The present invention is directed to methods and kits for inactivation of mycobacteria. In one embodiment, the method may include: transferring a sample from a liquid medium containing acid-fast bacteria to a first tube, the first tube having a body, an opening. And a second end of the body having a frustoconical portion ending with a concave tip; centrifuging the tube to pellet the mycobacteria into the concave tip; Decanting the supernatant; resuspending the mycobacterial pellet in alcohol; transferring the suspension to a second tube containing beads; a second to disrupt the mycobacterial cells Agitating the tubes; and incubating the suspension to inactivate acid-fast bacteria in the test sample. The method may also include the step of identifying acid-fast bacteria using mass spectrometry. [Selection] Figure 1