Identification of Phospholipid Molecular Species in Porcine Brain Extracts Using High Mass Accuracy of 4.7 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

摘要

Lipids play a critical role in many metabolic and biological processes,including energy production and storage,the formation and functioning of cellular membranes,and signal transduction.The biological importance of lipids has drawn extensive attention to the analysis of lipid molecular species.In particular,mass spectrometry (MS)has been shown to be a powerful tool in elucidating the diversity of lipid species and their participation in regulating cellular functions.In recent years,Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS)has been increasingly utilized,particularly in profiling global lipid distribution.Its unprecedented mass accuracy enables the discernment of mass differences of less than 0.01 mass unit,such as those often observed in lipid analyses.Under the assumption that the cation species are known,the minimum accuracy for assigning the chemical composition of lipid species is 21.38 ppm.