A method to purify, to detect and identify specific CDR peptides by Mass Spectrometry

摘要

CDR peptides could be detected down to an amount of 110 attomole, 5 orders of magnitude lower than the total IgG concentration. Using a combination of LTQ-Orbitrap CID and HCD fragmentation methods, we were able to correctly obtain a de novo sequence for approx50percent of the detected CDR peptides. Immune affinity capturing can be used to further increase the sensitivity of MS detection of CDR peptides. Specific CDR peptides could be detected and sequenced at relatively low levels which should allow the detection of clinically relevant CDR peptides in patient samples.