Determination of cyromazine and metabolite melamine in animal tissues by liquid chromatography tandem mass spectrometry

摘要

Solid-phase extraction (SPE) combine with liquid chromatography-mass spectrometry to determine the trace amount of cyromazine and melamine in chickens was developed. In the proposed method, the limit of detections of cyromazine and melamine were down to ng/g level. The feasibility of applying SPE-LC-MS/MS to determine the amount of cyromazine and melamine in real chickens. The concentrations of cyromazine and melamine were detected in the chickens in the range of 1 to 51 ng/g. The proposed method is sensitive enough to determine the trace of cyromazine and melamine in chicken samples.