Negative Ion Mode ESI-LC-MRM-MS for Differentiation of Native Human Milk Tetra- and Pentaoses Possible Application in Human-Milk-Typing

摘要

Our novel negative ion mode μESI-LC-MRM-MS method proofed to deliver unambiguous detection and distinction of isobaric Type I and Type II-core HM Tetraoses (LNT & LNnT) and 4 fucose-positional pentaose-isomers (LNFPI-V). Applicability of this concept to real world samples after minimal sample pretreatment was demonstrated and cross validated by HPAEC analyses. Since different human milk types exhibit characteristic patterns of individual LNFP- and LNT-isomers, rapid human milk typing may thus be facilitated.