Monitoring of H3K56ac level in cancer cell lines during cell cycle by SRM

摘要

On the level of the chromatin activity of gene expression is orchestrated by the posttranslational modifications of histones. Histone acetylation generally impairs nucleosomal stability. Nucleosomes containing acetylated lysine 56 on histone H3 (H3K56ac) remain stable. Instead, this modification promotes nucleosome disassembly in the process known as nucleosome breathing. Then the DNA is more susceptible to interaction with nuclear proteins. H3K56ac is a marker of newly synthesized nucleosomes during DNA replication and reparation or high nucleosome turnover in active gene promoter site in yeasts. In contrast to yeasts, in mammalian cells the H3K56ac is catalyzed by dissimilar histone acetyltransferases with much lower efficiency. Role of H3K56ac in nuclear processes and carcinogenesis in mammalian cells is still unknown. Ambiguous and distinct results in the H3K56ac research are caused by the antigen rarity in combination with cross-reactivity of antibodies. We focused on the regulation of H3K56ac during cell cycle and compared its level in mammalian cells by mass spectrometry based method.