Development of a paper spray mass spectrometry cartridge with integrated solid phase extraction for bioanalysis

摘要

The aim of this work was to create an "all-in-one" disposable capable so that sample extraction, pre-concentration, and ionization could be performed from a single device. Compared to direct paper spray, the integrated SPE approach: (1) improved the MS signal intensity and detection limits significantly; (2) have lower levels of ionization suppression and better overall recovery. The five drugs tested showed good linearity when an isotopically labeled internal standard was spiked into the sample. The simple and automatic on-cartridge preparation could also enable more effective use of portable mass spectrometers or the use of mass spectrometers outside traditional analytical labs.