An automated workflow for rapid alignment and identification of lipid biomarkers obtained from chip-based direct infusion nanoelectrospray tandem mass spectrometry

摘要

Lipid fingerprinting can be used for fast hypothesis generation. Chip-based nanoESI infusion can be used for cross-contamination free screening of plasma or tissue full extracts for phospholipids. Chip-based nanoESI infusion allows fast acquisition (one minute per sample) in high/low resolution mode. Iontrap technology together with MS/MS scans used for phospholipid identification via head group and neutral loss analysis of fatty acids identified around 50 lipid components. Expressionist Refiner MS workflow performs full data analysis in 20 seconds. Expressionist Analyst platform performs multivariate and univariate statistics. Perturbations in the phospholipid contents for rats exposed to environmental tobacco smoke (ETS) were observed.