Investigation of Site-Specific N-Glycosylation of Glycoprotein by Reversed-Phase Capillary LC Coupled with Electrospray Linear Ion Trap Mass Spectrometry

摘要

Protein ID software, Sequest, was used to determine peptide sequence and glycosylation site based on datadependent MS3 spectra of glycopeptide. This approach was applied to transferrin and bovine alpha-1 acid glycoprotein: N-glycosylation sites as well as sitespecific N-glycan profile were determined.