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Effect of Enhanced Prostacyclin Synthesis by Adenovirus-Mediated Transfer on Lipopolysaccharide Stimulation in Neuron-Glia Cultures

机译:增强前列腺素合成的腺瘤介导的转移对神经元 - 胶质胶质促进脂多糖刺激的影响

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Prostacyclin (PGI_2) is known as a short-lived, potent vasodilator and platelet anti-aggregatory eicosanoid. This work attempts to selectively augment PGI_2 synthesis in neuron-glia cultures by adenoviral (Ad) gene transfer of PGI synthase (PGIS) or bicistronic cyclooxygenase 1 (COX-1)/PGIS and examines whether PGI_2 confers protection against lipopolysaccharide (LPS) stimulation. Cultures released low levels of eicosanoids. Upon Ad-PGIS or Ad-COX-1/PGIS infection, cultures selectively increased prostacyclin release. Both PGIS- and COX-1/PGIS-overexpressed cultures contained fewer microglial numbers. Further, they significantly attenuated LPS-induced iNOS expression and lactate, nitric oxide, and TNF- production. Taken together, enhanced prostacyclin synthesis in neuron-glial cultures reduced microglia number and suppressed LPS stimulation.
机译:前列环素(PGI_2)被称为短寿命,有效的血管扩张剂和血小板抗聚糖型果索糖。该工作试图通过PGI合酶(PGI)或双顺铬环氧基酶1(COX-1)/ PGI的腺嘌呤(AD)基因转移选择性地增强PGI_2在神经元 - 胶质胶质培养物中的合成,并检查PGI_2是否赋予脂多糖(LPS)刺激的保护。培养物释放出低水平的果香糖。在Ad-PGI或Ad-Cox-1 / PGIS感染后,培养物选择性地增加前列腺素释放。 PGIS和COX-1 / PGIS过表达培养物含有较少的小胶质数。此外,它们显着减弱了LPS诱导的InOS表达和乳酸,一氧化氮和TNF-生产。一起服用,在神经元胶质培养物中增强前列环素合成减少了微胶质细胞数量并抑制了LPS刺激。

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