Study on Separation, Purification and Functional Properties of Goose Plasma Transferrin

摘要

The content of globins was determined by cellulose acetate film electrophoresis. The goose plasma trasferrin was separated from goose blood by ion-exchange on DEAE-52 and sephadex gel on G-100. The ability of apo-Tf and holo-Tf to damage the selected Gram-negative bacteria and the influence of Fe~(3+) on antibacterial ability of transferring were studied. It was found that β globulin was the major part of goose plasma globins and the content of β globulin was 34. 809 mg/dl. The goose transferrin has antimicrobial activity against select Gram-negative bacteria. The molecular weight of Transferrin is 77 KD by SDS-PAGE. The characteristic absorb peak of Transferrin is 470nm. Experimental results predict that Fe~(3+) changes the structure of transferrin, and this change reduce the ability of transferrin.