PRODUCTION OF FRUCTO-OLIGOSACCHARIDES FROM SUCROSE SYRUP BY A MICROORGANISM TSC-FOS1

摘要

Fructosyltransferase activity from a microorganism (designated TSC-FOS1) capable of converting sucrose to fructo-oligosaccharide (FOS) was investigated. Using cultured cells, we were able to produce FOS from 50% sucrose syrup. Maximum cellular activity of FOS production was determined to be pH 6-7 and 65 °C. Enzyme activity was stable within a very narrow pH range, pH 5; and it was sensitive to higher temperature. Optimum reaction condition for FOS production was determined to be at pH 5.5 and 55°C, after considering stability factors. The activity was inhibited by FeCl_3, Al_2O_3, and methanol. The FOS content produced by the cultured cells was between 55-60% (FOS to total carbohydrates).