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The purification and structure of taste-active peptide in water-soluble extraction of Parma ham

机译:帕尔马火腿水溶性提取中味觉活性肽的纯化和结构

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Fractions of the taste-active peptide (G-25-P4-r1) were obtained by gel filtration chromatography, semi-preparative RPHPLC chromatography and analytical RP-HPLC chromatography from the water-soluble extract (WSE) of Parma ham. The peptide's amino acid sequence, Ala-His-Ser-Val-Arg-Phe-Tyr, was gained using MS/MS analysis. It was also synthetic produced and the taste activity was verified with the similar taste to the WSE by electronic tongue and sensory evaluation.
机译:通过凝胶过滤色谱法,半制备型RPHPLC色谱法和分析型RP-HPLC色谱法从帕尔马火腿的水溶性提取物(WSE)中获得味觉活性肽(G-25-P4-r1)的级分。使用MS / MS分析获得了肽的氨基酸序列Ala-His-Ser-Val-Arg-Phe-Tyr。它也是人工合成的,并且通过电子舌头和感官评估以与WSE相似的口感验证了口感活性。

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