Light Addressable Potentiometric Sensor (LAPS) Integrated Microfluidic System for Real-time Cell Acidification Detection

摘要

Cell metabolism is a common biological mechanism in living cells. Acidification plays an important role in cell metabolism, in which the extracellular pH change is used to indicate the vitality of cells. For extracellular detection of cell metabolic substances, light addressable potentiometric sensor (LAPS) has many advantages, such as high sensitivity, easy encapsulation, and convenient integration with microfluidic system for cell experiments. LAPS is a spatially resolved biochemical sensor based on field-effect. Characteristic test experiments and cell metabolism experiments were carried out to determine the performance of LAPS by monitoring the pH change of hepatoma HepG2 cells. The pH sensitivity is 51.18mV/pH, and the extracellular pH of HepG2 cells changes from 7.19 to 6.28 after high glucose (125mM) medium was injected. The results showed that the microfluidic system presented good performance in real-time detection of cell acidification.