Light-Addressable Potentiometric (LAP) Sensor Assay of Newcastle Disease Virus

摘要

A sandwich immunoassay for Newcastle Disease Virus (NDV) was developed using streptavidin-biotin mediated filtration capture and silicon based semiconductor detection. The assays were carried out in one step whereby the immunoreagents and the antigen (NDV) were mixed together and incubated. The pH sensing capability of the detector, a light addressable potentiometric (LAP) sensor, was employed to detect the presence of immobilized urease-conjugated antibodies. Lower limits of detection (LOD) of the assay were determined as a function of incubation time. The LOD were approximately 27,15, and 3 ng per well for 1, 5, and 60 minute incubations, respectively. The techniques described here have the potential to be adapted to an automated LAP sensor for use in the Biochemical Detector program.