METHODS FOR DETECTING ANALYTES USING A GRAPHENE-BASED BIOLOGICAL FIELD-EFFECT TRANSISTOR

摘要

Methods for detecting analytes using a biological field-effect transistor (BioFET) are disclosed. In some implementations, the method includes exposing a three-dimensional (3D) graphene layer biofunctionalized with a biological recognition element to a target analyte, providing a well region containing an electrolyte solution configured to retain the target analyte, allowing the target analyte to disperse throughout the electrolyte solution and bind with the biological recognition element, detecting a change in electrical properties of the 3D graphene layer in response to the target analyte binding with the biological recognition element, determining a presence of the target analyte based on the change in electrical properties, and outputting an indication of the determined presence of the target analyte. In some aspects, the 3D graphene layer may operate as a channel for the BioFET.