IMMUNOFLUORESCENCE KIT FOR DETECTING PD-L1 EXPRESSION OF PERIPHERAL BLOOD CIRCULATING TUMOR CELLS OF KIDNEY CANCER PATIENT AND DETECTION METHOD

摘要

An immunofluorescence kit for detecting a PD-L1 expression of peripheral blood circulating tumor cells of a kidney cancer patient and a detection method. The kit comprises 45 mL of diluent, 1 mL of destaining solution, 0.5 mL of staining solution A, 1 mL of staining solution B, 200 μl of methanol, 200 μl of 2% PFA, 100 μl of 10% goat serum, 100 μl of primary antibody suspension composed of mouse anti-CK, rat anti-CD45 and rabbit anti-PD-L1, 100 μl of secondary antibody suspension composed of a fluorescently labeled goat anti-mouse, a fluorescently labeled goat anti-rat and a fluorescently labeled goat anti-rabbit, and a DAPI mounting medium. According to the detection method, the PD-L1 expression condition of a patient with advanced or recurrent kidney cancer can be detected without acquiring a tissue specimen by means of needle biopsy.