Rapid Detection of HLA Genes Expression in Peripheral Blood Mononuclear Cell of Gastric Cancer Patients by Reverse Transcriptase Polymerase Chain Reaction

摘要

To investigate the expression of HLA gene mRNA in peripheral blood mononuclear cell (PBMC) of patients with gastric cancer. HLA-A, -B, -DP, -DR, -G mRNA expression levels in 43 patients and 22 healthy individuals were performed by reverse transcriptase polymerase chain reaction(RT-PCR) and real-time RT-PCR. The expressions of HLA-A, -B,-G mRNA in gastric cancer group were significantly lower than that in healthy group, while there was no significant difference in HLA-DP, -DR between two groups. The expression of HLA-A -B and -G in PBMC weren't associated with the clinical staging and histological grading of gastric cancer. While the expression of HLA-G in III + IV grade was lower than that in I+ II grade. HLA-A, -B, -G may play critical roles in the tumor immune response. RT-PCR or real-time PCR may be used as a rapid and simple method to detect HLA gene mRNA expression.