Method for in vivo high-throughput evaluating of RNA-guided nuclease activity

摘要

The present invention relates to a method for evaluating the activity of RNA-guided nuclease in cells in a high-throughput manner, and specifically, a guide RNA coding nucleotide sequence and a target It relates to a method for evaluating the activity of an RNA-guided nuclease from the indel frequency of a cell library, including an isolated oligonucleotide comprising a nucleotide sequence. The method for characterizing RNA-guide nucleases using the guide RNA-target sequence pair library of the present invention is RNA-guided nuclea in a high-throughput manner in cells (in vivo). Since it enables the evaluation of the activity of the agent, it can be very useful in all fields of applying RNA-guided nucleases.