in vivo RNA- Method for in vivo high-throughput evaluating of RNA-guided nuclease activity

摘要

The present invention relates to a method for evaluating RNA-guided nuclease activity in cells in a high-throughput manner, and specifically, to a method for evaluating RNA-guided nuclease activity from the indel frequency of a cell library, including separated oligonucleotides including coding nucleotide sequences of guide RNA and target nucleotide sequences. According to the present invention, the method for analyzing the characteristics of RNA-guided nucleases using the library of guide RNA-target sequence pairs is an in vivo high-throughput method that allows evaluation of RNA-guided nuclease activity, and therefore, the method can be effectively utilized in all fields where RNA-guided nucleases are applied.